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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
02/03/2017 |
Actualizado : |
07/03/2017 |
Autor : |
GARCIA, J.P; GIANNITTI, F.; FINNIE, J.W.; MANAVIS, J. .; BEINGESSER, J.; ADAMS ,V.; ROOD, J.I.; UZAL, F.A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Comparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Veterinary Pathology, 2015, v.52.n.6. p.1250-1253, 2015. |
Idioma : |
Inglés |
Contenido : |
Abstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. MenosAbstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated wi... Presentar Todo |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
Asunto categoría : |
-- |
Marc : |
LEADER 02512naa a2200217 a 4500 001 1056750 005 2017-03-07 008 2015 bl uuuu u00u1 u #d 100 1 $aGARCIA, J.P 245 $aComparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives.$h[electronic resource] 260 $c2015 520 $aAbstract Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. 650 $aENFERMEDADES DE LOS ANIMALES 700 1 $aGIANNITTI, F. 700 1 $aFINNIE, J.W. 700 1 $aMANAVIS, J. . 700 1 $aBEINGESSER, J. 700 1 $aADAMS ,V. 700 1 $aROOD, J.I. 700 1 $aUZAL, F.A. 773 $tVeterinary Pathology, 2015$gv.52.n.6. p.1250-1253, 2015.
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
Fecha actual : |
18/09/2014 |
Actualizado : |
25/11/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
A - 1 |
Autor : |
CASTILLO, A.; REBUFFO, M.; DALLA RIZZA, M.; FOLLE, G.; SANTIÑAQUE, F.; BORSANI, O.; MONZA, J. |
Afiliación : |
ALICIA MARIA CASTILLO SALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Generation and characterization of interspecific hybrids of Lotus uliginosus × Lotus corniculatus. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Crop Science, 2012, v. 52, no. 4, p. 1572-1582. |
ISSN : |
0011-183X |
DOI : |
10.2135/cropsci2011.07.0374 |
Idioma : |
Inglés |
Notas : |
Article history: Published July, 2012. |
Contenido : |
ABSTRACT.
A method is described for obtaining interspecific hybrids between commercial lines of Lotus uliginosus Schkuhr and Lotus corniculatus L. Hybridization was possible between these species using embryo rescue. Two strategies were used to confirm 40 F1 hybrids, that is, flow cytometry and microsatellite simple sequence repeats (SSRs). The L. uliginosus and L. corniculatus parents have significantly different genome size (2.5 ± 0.02 and 2.22 ± 0.02 pg, respectively) and the offspring from the cross had intermediate values. Two SSR primers were polymorphic between the genotypes tested, TM1150 and EH380069. Maternal effect was detected in L. uliginosus genotypes, as determined by greater frequency of F1 hybrids. Recombinant traits were observed in the F1 hybrid progeny. Ninety F1 hybrids resulting from L. uliginosus × L. corniculatus crosses showed different levels of fertility in the polycross; <3% F1 plants produced more than 1000 seeds without embryo rescue while 10% of plants did not flower the first year. The F1 hybrid plants grown under greenhouse conditions exhibited shoot phenotypes similar to L. uliginosus (maternal) whereas F2 phenotypes were similar to L. corniculatus (paternal) in field trials. Rhizome presence was observed in 60% of F2 hybrid plants. The diameters of the root crowns of these plants, however, were similar to that of L. corniculatus.
© Crop Science Society of America. |
Palabras claves : |
LEGUMINOSAS FORRAJERAS; MARCADORES MOLECULARES. |
Thesagro : |
CULTIVOS; PLANTAS FORRAJERAS. |
Asunto categoría : |
-- F01 Cultivo |
Marc : |
LEADER 02262naa a2200277 a 4500 001 1050609 005 2019-11-25 008 2012 bl uuuu u00u1 u #d 022 $a0011-183X 024 7 $a10.2135/cropsci2011.07.0374$2DOI 100 1 $aCASTILLO, A. 245 $aGeneration and characterization of interspecific hybrids of Lotus uliginosus × Lotus corniculatus.$h[electronic resource] 260 $c2012 500 $aArticle history: Published July, 2012. 520 $aABSTRACT. A method is described for obtaining interspecific hybrids between commercial lines of Lotus uliginosus Schkuhr and Lotus corniculatus L. Hybridization was possible between these species using embryo rescue. Two strategies were used to confirm 40 F1 hybrids, that is, flow cytometry and microsatellite simple sequence repeats (SSRs). The L. uliginosus and L. corniculatus parents have significantly different genome size (2.5 ± 0.02 and 2.22 ± 0.02 pg, respectively) and the offspring from the cross had intermediate values. Two SSR primers were polymorphic between the genotypes tested, TM1150 and EH380069. Maternal effect was detected in L. uliginosus genotypes, as determined by greater frequency of F1 hybrids. Recombinant traits were observed in the F1 hybrid progeny. Ninety F1 hybrids resulting from L. uliginosus × L. corniculatus crosses showed different levels of fertility in the polycross; <3% F1 plants produced more than 1000 seeds without embryo rescue while 10% of plants did not flower the first year. The F1 hybrid plants grown under greenhouse conditions exhibited shoot phenotypes similar to L. uliginosus (maternal) whereas F2 phenotypes were similar to L. corniculatus (paternal) in field trials. Rhizome presence was observed in 60% of F2 hybrid plants. The diameters of the root crowns of these plants, however, were similar to that of L. corniculatus. © Crop Science Society of America. 650 $aCULTIVOS 650 $aPLANTAS FORRAJERAS 653 $aLEGUMINOSAS FORRAJERAS 653 $aMARCADORES MOLECULARES 700 1 $aREBUFFO, M. 700 1 $aDALLA RIZZA, M. 700 1 $aFOLLE, G. 700 1 $aSANTIÑAQUE, F. 700 1 $aBORSANI, O. 700 1 $aMONZA, J. 773 $tCrop Science, 2012$gv. 52, no. 4, p. 1572-1582.
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